Compact single fiber optical tweezer–micropipette system for completely noninvasive cell sorting

Bridging optical tweezers and microfluidics can form a multifunctional platform, which overcome the difficulties of precise manipulation in hydrodynamic flow noninvasive method. However, when integrated into microfluidic chip, fiber optic tweezer loses its flexibility. Here, we propose compact single tweezer–micropipette system. It sort particles by differences shape refractive index completely way while retaining flexibility, high selectivity, precision tweezer. Compact channels are formed combining two different-diameter micropipettes. The internal diameter circular channel is less than 30 μm. Furthermore, calculated trapping pushing regions Yeast Chlorella achieved separation from experiments. We did 90 sets tests on sorting accuracy (SFOT). SFOT was able to distinguish between types each test. With advantages accuracy, low power consumption, structure, such methods be used fields separation, cell transportation, sorting, single-cell analysis.